P-053 Sperm aneuploidy and DNA integrity pre- and post-chemotherapy in men with testicular cancer

Abstract Study question Are fragmentation levels (single-double stranded DNA breaks) or aneuploidy frequencies in sperm increased patients with testicular cancer who recovered spermatogenesis after chemotherapy (CT)? Summary answer Similar of and frequency are found men pre- post-chemotherapy. What is known already Testicular the most frequent solid tumor among young men. Therefore, restoration fertility achievement fatherhood survivors have become important concerns. The integrity genetic material crucial since some paternal genes essential for early embryo development. Although previous studies analyzed effect CT on integrity, they did not differentiate single vs. double-stranded breaks. Few data available from survivors, so it remains to be elucidated whether treatments may lead an risk syndromes offspring. design, size, duration Prospective multicenter study including 31 males a history requested semen cryopreservation between 2007-2021. All included underwent once treatment ended. Post-CT samples were collected seminal parameters, single-double breaks proportion aneuploidies compared one aliquot initial ejaculate. project was approved by Institutional Review Board written informed consent participation obtained each subject. Participants/materials, setting, methods Patient´s age prior 31.23yrs (IC 95%: 29.29-33.17). Mean interval time 1st 2nd 4.53yrs 3.42-5.64). TUNEL quantified overall flow cytometry analyzing at least 20,000 cells, while γH2AX staining applied double assessment. Sperm calculated using fluorescence situ hybridization (FISH) probes chromosomes 13, 18, 21, X, Y. A paired t-test used compare variables post-CT. Main results role chance when quality parameters compared. concentration pre-CT 33.03M/mL 23.98-42.07) 36.41 M/mL 27.85-44.96) post-CT ejaculates (N.S.). comparable rate motile spermatozoa observed [(43.81% 38.42-49.19) 44.73% 38.95-50.51), N.S.]. Regarding assay results, 21.65% 17.66-25.63) 23.00% 19.90-26.61) Furthermore, similar (H2AX) pre [10.2% 6.45-13.96) 12.44% 8.75-16.13) Comparable disomic proportions PRE POST-CT samples: [(Cr.13 =0.02%, Cr.18 =0.01%, Cr.21=0.30%, Cr.XY=0.84%) (Cr.13 =0%, Cr.21=0%, Cr.XY=0.33%), N.S]. Results also evaluated according elapsed POST -CT samples. No significant differences values (sperm concentration, p = 0.26; motility, 0.68), (p double-strand 0.90) disomies (Cr. 13 0.79, 0.46, Cr. 21 0.85, Cr.XY 0.05). Limitations, reasons caution main limitations present that we only recruited our long-term CT. Wider implications findings wishing their own children gonadotoxic should receive adequate counseling about side effects therapy. Based euploidy, advised use preserved before undergoing Trial registration number na